胰岛素检测试剂盒

ag线上真人游戏怎么刷流水 是德国DRG的代理商，提供优质的胰岛素检测试剂，分别可检测大鼠胰岛素、小鼠胰岛素和人胰岛素，本文介绍的胰岛素INS检测试剂盒（糖代谢）货号为EIA2935是一款科研用途试剂盒，定量检测适用于科研工作，该试剂盒采用ELISA方法进行检测。该操作方法简单方便，灵敏度高特异性强的特点，可同时检测96人份节省时间成本，酶标仪自动读取数据。检测结果准确。下面主要介绍三个检测物种的胰岛素检测试剂的介绍和参考资料。

胰岛素介绍

胰岛素是由*内的胰岛β细胞受到内源性或外源性的物质刺激，如葡萄糖、乳糖、核糖、精氨酸、胰高血糖素等的刺激而分泌的一种蛋白质激素。它是人体内*的具有降糖作用的激素。胰岛素是机体内*降低血糖的激素，同时促进糖原、脂肪、蛋白质合成。

其主要的生理作用：1、调节糖代谢：促进全身组织摄取和利用葡萄糖；2、调节脂肪代谢：促进脂肪合成和贮存，减少血液中游离脂肪酸；3、调节蛋白质代谢：促进氨基酸摄取和蛋白质合成，抑制蛋白质分解。外源性的胰岛素可以用于治疗糖尿病。

检测原理：

DRG公司的这一款胰岛素INS检测试剂盒（糖代谢）检测方法是酶免法，这一实验是一种基于夹心法原理的固相酶联免吸附实验(ELISA),反应板上的微检测孔包被有能结合胰岛素分子上特异性的单克隆抗体。一份含有内源性胰岛素的病人样本在包被孔中与酶联物进行孵育，该酶联物是一种生物素标记的抗胰岛素抗体。孵育后，用洗涤液洗去未结合的酶联物。在第二次温育时，链亲和素过氧酶复合物结合到生物素化抗胰岛素抗体上，过氧酶复合物的结合量与样品中胰岛素的浓度成正比。加入底物液后，所显示的颜色强度与病人样品中胰岛素的浓度成正比

【试剂盒组成成分】

1) 微孔板：12×8，96孔，包被抗胰岛素的单克隆抗体。

2) 零标准品 ：1支，3ml，即用型，0μIU/ml。

3) 标准品1－5：5支，1ml，即用型，6.25;12.5;25;50;100μlU/ml。

4) 酶联物：1支，5ml，即用型，生物素化的鼠单克隆抗体。

5) 酶复合物：1支，7ml，即用型，辣根过氧化物-链亲和素复合物。

6) TMB底物液：1支 14ml 即用型。

7) 终止液： 1支 14ml 即用型 包含0.5M硫酸,请勿接触终止液，以免刺激或着烧皮肤。

8) 洗涤液：1瓶，30ml，40倍浓缩，见试剂准备。

人胰岛素检测试剂盒

小鼠胰岛素检测试剂盒

大鼠胰岛素检测试剂盒

ag线上真人游戏怎么刷流水 还提供其他糖尿病检测试剂盒，胰岛素样生长因子-1、C肽、胰岛素抗体、IGF-1-600、GIP-1等等，欢迎咨询我们获取资料。

Summary and Explanation Insulin is the principal hormone responsible for the control of glucose metabolism. It is synthesized in the β-cells of the  islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in  equimolar amounts into the portal circulation. The mature insulin molecule comprises two polypeptide chains, the A chain  and B chain (21 and 30 amino acids respectively). The two chains are linked together by two inter-chain disulphide  bridges. There is also an intra-chain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration, and the hormone has a number of important  metabolic actions. Its principal function is to control the uptake and utilisation of glucose in peripheral tissues via the  glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and  glycogenolysis are counteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline), growth  hormone and cortisol. Insulin concentrations are severely reduced in insulin-dependent diabetes mellitus (IDDM) and some other conditions  such as hypopituitarism. Insulin levels are raised in non-insulin-dependent diabetes mellitus (NIDDM), obesity, insulinoma  and some endocrine dysfunctions such as Cushing’s syndrome and acromegaly。

The DRG Insulin ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich  principle.  The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site on the Insulin  molecule.  An aliquot of patient sample containing endogenous Insulin is incubated in the coated well with enzyme conjugate, which  is an anti-Insulin antibody conjugated with Biotin. After incubation the unbound conjugate is washed off.  During the second incubation step Streptavidin Peroxidase Enzyme Complex binds to the biotin-anti-Insulin antibody. The  amount of bound HRP complex is proportional to the concentration of Insulin in the sample.  Having added the substrate solution, the intensity of colour developed is proportional to the concentration of Insulin in the patient sample.

胰岛素INS检测试剂盒（糖代谢）定量检测，灵敏度高特异性好，适用于高校科研工作，您可以在此平台上给我们留言，或者关注“ag线上真人游戏怎么刷流水 "公众号与我们客服联系。